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FuGENE® HD is a 100% synthetic, multi-component, non-liposomal transfection reagent designed for the delivery of DNA into more challenging eukaryotic and insect cell lines. FuGENE® HD interacts with nucleic acids & the cell’s membrane to provide efficient and safe entry into the cell. This mechanism allows users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells, and has successfully been used to transfect over 1000 cell lines. With a protocol that is straight-forward and quick, FuGENE® HD allows researchers and scientists to free up valuable lab time.
HCT116 Cells were seeded in 96-well plates according to user guides and subsequently transfected with FuGENE® HD or Lipofectamine® 3000 (registered trademark of Life Technologies Corporation)+ GFP expressing plasmid at recommended 3:1 ratios. Cells were then analyzed via flow cytometry at 24, 48, and 72 hours post-transfection to measure +GFP expressing cells and total cell viability (propidium iodide). Graphs above show the superior performance of FuGENE® HD vs. Lipofectamine® 3000 in HCT116 cells.
A549, COS7, HCT116, HEK293, and HeLa cells were seeded in 96-well plates according to users guide and subsequently transfected with FuGENE® HD Reagent & GFP expressing plasmid at various FuGENE® HD to Total DNA ratios. Cells were then analyzed after 24 hours for transfection efficiency utilizing flow cytometry. Data for normalized GFP expression is plotted in the graph to left highlighting the best FuGENE® HD to DNA ratio for each cell line tested.