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Harnessing the same gentle reliability as our market-leading DNA Transfection Reagents, we are proud to introduce to you FuGENE® SI, an innovative RNA Transfection reagent engineered for the delivery of siRNA. FuGENE® SI is a 100% synthetic, multi-component transfection reagent designed for the delivery of RNA molecules into eukaryotic cell lines. FuGENE® SI allows researchers to obtain maximum efficiency knockdowns while being more gentle on cells than competitor’s reagents. Request a sample today for your RNA delivery needs and See the Unseen® with FuGENE® SI.
HEK293-GFP & NIH3T3-GFP Cell lines (Cell Biolabs®) were seeded in 96-well plates according to user guides and subsequently transfected with 0.2, 1.0, or 5 pmols of GFP targeting siRNA or negative control (Horizon Discovery/Dharmacon®), along with 0.3ul of Transfection Reagent FuGENE® SI or 0.3ul of Lipofectamine® RNAiMax (Life Technologies Corporation). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % knockdown of GFP & total cell viability. Graphs above show the superior knockdown performance of FuGENE® SI vs. Lipofectamine® RNAiMax in HEK293 and NIH3T3 cells.