Frequently Asked Questions
What reagent do you suggest for stem cell research?
FuGENE® 6, HD, and 4K have all been utilized in Stem Cell Research. If you have questions about a particular cell line, the citation database on our website is a great resource (www.fugene.com/citations-database/). Search by key attributes such as cell type, application, product line, or year of publication. If you still feel unsure, contact our technical team at contact@fugene.com.
How is FuGENE® 4K chemically different from Lipofectamine® 3000?
FuGENE® 4K is a multi-component blend of polymers and lipids that allows high efficiency transfection while remaining gentler on your cells than Lipofectamine® 3000. In addition, 4K is capable to producing high yields of Lenti Virus using most any type of HEK293 cell and multiple types of growth media.
Does Promega or Roche manufacture FuGENE® reagents?
No, only FuGENE manufactures FuGENE reagents. To better serve our customers, you can now order our products directly through this web site or through our many distributors. Regarding Roche and Promega; in 2010 Promega replaced Roche as our World Wide distributor.
What do you suggest if I am having a hard time transfecting my cells with FuGENE® Transfection Reagents?
We suggest you check our Resource page and if still having trouble contact us directly through this site. We are always here to help.
What is new about FuGENE® 4K and FuGENE® SI compared to FuGENE® 6 and FuGENE® HD?
FuGENE® 6 and HD are our legacy reagents that have been out on the market for quite some time. In the last two years FuGENE® has been innovating new reagents. FuGENE® 4K was launched in early 2022 and is our most advanced DNA Transfection reagent for high efficiencies and low toxicities. FuGENE® SI was launched in mid-2021 and is our first RNA transfection reagent optimized for siRNA delivery. It enables researchers to obtain robust gene knockdowns while remaining gentle on your cells.
Who do we contact for customer or technical support?
Please contact us directly at contact@fugene.com and you will receive a response within 24 hours.
Do you offer bulk or custom formulations?
We pride ourselves on being nimble for our customers needs and certainly welcome any custom or bulk formulation requests. With a dedicated PhD team of biologists and chemists we are ready to tackle any project.
How can we get help optimizing for our cell line and application?
Navigate to our Documents/Manual page for a handy optimization guide, or check out our Basics in Transfection Page. If those are not sufficient, then please email us at contact@fugene.com for our expert technical assistance.
How do you select the best DNA Transfection reagent for your cell lines amongst FuGENE® products?
We suggest you first see if your cell line is in our citation database found on the resource page. For unique applications we suggest you contact us. We will reply in 24 hours.
Do you offer product samples?
Yes. Please see our resource page on how to obtain samples.
How can FuGENE® be purchased?
All FuGENE® Transfection reagents can be purchased directly through this web site or via one of our distributors.
Who manufactures FuGENE® Transfection Reagents?
Only our main facility located in Madison, Wisconsin has ever manufactured genuine FuGENE Products.