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FuGENE® 4K Transfection Reagent

SKU: N/A Category: FuGENE 4K® Tag: DNA

$538.00

For phone orders, international or bulk orders, please reach out at contact@fugene.com or by phone at +1(608) 628-7772.

Product Information

Best-in-Class DNA Transfection Reagent

FuGENE^® 4K is our newest and most advanced, 100% synthetic, multi-component transfection reagent designed for the delivery of DNA into both challenging and routine mammalian cell lines. FuGENE® 4K was developed to improve upon our widely published and trusted FuGENE^® HD reagent. Leveraging our gentle and highly efficient proprietary transfection chemistry, FuGENE^® 4K provides efficient and safe entry of DNA into a wide range of cells. This mechanism allows users to obtain higher performance than other competitor reagents including Lipofectamine™ 3000.

benefits

Benefits of FuGENE® 4K

Outperforms all other reagents for Lenti Virus production, even in suspension cells
Room Temperature storage
Superior transfection efficiencies compared to competitors
Increased performance when compared to Lipofectamine™ 3000
Transfect in serum-free or serum-containing media
Quick and easy protocol with limited optimization steps
Transfect difficult-to-transfect mammalian cell lines
Improved performance in the most relevant cancer cell lines
Maximizes protein production in 293 & CHO cells

What some of our customers have said:

Comparison Chart

FuGENE 4K vs. Lipofectamine™ 3000

FuGENE® 4K reagent outperforms Lipofectamine™ 3000. A549, C2C12, HCT116, CHO-K1, HeLa, NIH3T3, LNCaP, PANC1 & SKOV3 cell lines were seeded in 96-well plates according to user guides and subsequently transfected with FuGENE® 4K or Lipofectamine™ 3000 (registered trademark of Thermo Fisher Scientific) and a GFP expressing plasmid at the following transfection reagent to DNA ratios (3:1, 4:1 & 5:1). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % GFP expressing cells and % total cell viability (via propidium iodide dye). The graphs above show the superior performance of FuGENE® 4K vs. Lipofectamine™ 3000.

Comparison Benefits

Benefits of FuGENE 4K vs. Lipofectamine™ 3000

Performance

Obtain higher transfection efficiencies with FuGENE® 4K compared to Lipofectamine™ 3000.

Ease of Use

FuGENE® 4K is a single reagent kit, while Lipofectamine™ requires addition of separate reagent.

Cell Viability

FuGENE® 4K has comparable cell viability as Lipofectamine™ across most cell lines.

Cost Savings

Less cost per mL of FuGENE® 4K vs. Lipofectamine™ 3000.

Faster Protocol

FuGENE® 4K forms complex with DNA in as little as 5 minutes, and does not require additional component added to the transfection mix which requires preparation of an additional tube.