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FuGENE® 4K is our newest and most advanced, 100% synthetic, multi-component transfection reagent designed for the delivery of DNA into both challenging and routine mammalian cell lines. FuGENE® 4K was developed to improve upon our widely published and trusted FuGENE® HD reagent. Leveraging our gentle and highly efficient proprietary transfection chemistry, FuGENE® 4K provides efficient and safe entry of DNA into a wide range of cells. This mechanism allows users to obtain higher transfection efficiences than other competitor reagents. Whether it’s for difficult-to-transfect lines such as primary cells, stem cells, or suspension adapted 293 or CHO cells, our quick & easy FuGENE® 4K protocol allows users to obtain superior results and free-up valuable lab time.
FuGENE® 4K reagent outperforms Lipofectamine® 3000. A549, C2C12, HCT116, CHO-K1, HeLa, NIH3T3, LNCaP, PANC1 & SKOV3 cell lines were seeded in 96-well plates according to user guides and subsequently transfected with FuGENE® 4K or Lipofectamine® 3000 (registered trademark of Thermo Fisher Scientific) and a GFP expressing plasmid at the following transfection reagent to DNA ratios (3:1, 4:1 & 5:1). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % GFP expressing cells and % total cell viability (via propidium iodide dye). The graphs above show the superior performance of FuGENE® 4K vs. Lipofectamine® 3000.